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In a series of preliminary experiments, curde plasma membranes were prepared by centrifugation of ovarian and uterine homogenates at various centrifugal force from 3000 to 9000 xg. As expected, the recovery of the receptor increased with increased rotor speed, membranes were subsequently prepared by centrifugation at 9000xg in order to obtain maximal recovery of the receptor. The stability of the crude membrane receptor was markedly improved by supplementation of the buffer with glycerol; the hormone binding activity of the membrane extract was completely retained after storage at 4C for at least 3 day. The proteolysis of the receptor (and/or nicking), by endogenous protease, had been inhibited by the inclusion of an inhibitor cocktail containing PMSF, and EDTA in extraction buffer. Essay Writing Service - EssayEruditecom ABOUT US We value excellent academic writing and strive to provide outstanding essay writing services each and every time you place an order We write essays
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